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Weak Antimicrobial Activity of Ethanolic and Aqueous Extracts of Red Rambai (Lepisanthes alata)

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Red rambai (Lepisanthes alata) is an underulitised and increasingly rare tropical forest fruit. This fruit belongs to a family of Sapindaceae, but is not seasonal. The fruit skin is traditionally used as a stomach pain reliever. This study aimed to test the anti-microbial activity of ethanolic and aqueous extracts of red rambai fruit skin infusion against Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae. A total of 0.5, 1.0, or 1.5 mg of each extract, dissolved in 50 μl of sterile distilled water, was added to the test wells on a petri dish containing the assessed microbial. A concentration of 0.5 mg of tetracycline was used as a positive control for Escherichia coli and Staphylococcus aureus. Ketoconazole at concentration of 0.5 mg/50μl was ulitised as a positive control for Saccharomyces cerevisiae. A negative control was 50 μl of sterile distilled water. The diameters of inhibition zone were measured after incubation for 1 day at 37 °C. Weak antimicrobial properties was observed from the ethanolic and aqueous extracts of the red rambai fruit skin. The highest inhibition zone was observed from the highest ethanolic extract concentration, resulting in 2.05 ± 0.5 mm (22.5% positive control) against E. coli, 0.02 ± 1.89 mm (18.9% positive control) against S. aureus, and 1.08 ± 0.03 mm (21% positive control) against S. cerevisiae. It can be concluded that the efficacy of anti stomachache of red rambai fruit skin infusion was not caused by the antimicrobial properties.

Keywords: Red rambai fruit skin, infusion, Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae.

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Judul Seri

Proceeding. International Conference on Food, Agriculture, and Culinary Tourism. Samarinda, 4-6 August 2015

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Prosiding

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Samarinda : Jurusan THP Unmul., 2015

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Bahasa

English

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Prosiding

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Proceeding. International Conference on Food, Agriculture, and Culinary Tourism. Samarinda, 4-6 August 2015

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